Coding

Part:BBa_K4335003:Design

Designed by: Yuning Luo   Group: iGEM22_UESTC-BioTech   (2022-09-30)


ChlamyHgR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 782
    Illegal NgoMIV site found at 890
  • 1000
    COMPATIBLE WITH RFC[1000]

BBa_K4335003 is a resistance gene optimized for Chlamydomonas reinhardtii based on the original gene. We inserted a 145bp intron into the original gene to facilitate its expression in Chlamydomonas reinhardtii.

Design Notes

In order to make it easier to heterologously express in Chlamydomonas reinhardtii, we have optimized the codon and added a segment of the intron.


Source

Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-Finger Nucleases and CRISPR/Cas9. Plant Cell 29, 2498-2518. Hirano, M., Ando, R., Shimozono, S., Sugiyama, M., Takeda, N., Kurokawa, H., Deguchi, R., Endo, K., Haga, K., Takai-Todaka, R., et al. (2022). A highly photostable and bright green fluorescent protein. Nat Biotechnol 40, 1132-1142.

References